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Preclinical Studies and Early Drug Development: Predicting Tumor Response to Cytotoxic Therapy

Non-proliferation of MC38 fibrosarcoma cells following in vitro exposure to novel bacillus metabolized lactones

Morgan State University, Baltimore, MD, Howard University, Washington, DC, University of the West Indies, Kingston, Jamaica, Tai Sophia School of the Healing Arts, Laurel, MD, University of the West Indies, Kingston, Jamaica, NCI/NIH, Bethesda, MD

Abstract

B16

Introduction: Cancers are increasingly becoming an economic burden on health care systems. This is especially evident in developing economies. High medical cost has resulted in dependence on herbal therapies. Here we investigated the effects of an extract containing lactones obtained from the recently identified Bacillus mojavensis strain C14135 [GenBank] on MC38 fibrosarcoma cells. Materials/Methods: MC38 MCA Induced Fibrosarcoma cells were cultured in tissue culture flasks containing RPMI 1640 HyQ® media supplemented with 10%FBS, Penn/Strep and Amphotericin B. The flasks were incubated at 37^oC, 5% CO₂ and controlled RH. Cells were grown to 95% confluence then treated with .20ml and .50ml of .22µm filtered purified extract. The extract contained approx. 5.0µg/ml of the active principle. One flask remained as control. The flasks were incubated, and checked at 12hr, 24hr and 48hr interval. Results: Microscopic analysis of cells revealed extensive cellular damage. Dye exclusion analysis using Trypan blue also showed significant cell mortality. We employed Mitochondria Dehydrogenase (MDS) assay, which confirmed prior results. We report what appears to be an aggregation of cells, followed by detachment from the substrate and eventual cellular destruction. Conclusion: The results appear to demonstrate that compounds within this protein fraction are potentially catatonic to MC38 cancer cells. This phenomenon appears to be unique and possibly a result of innate compounds including Pyrrolo [1,2-a] piperazine-3,6-dione. Further experiments will be conducted to identify each compound's involvement in the established activity. Experiments will also include comparing results against present antineoplastic agents.